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The following antibodies were used: anti-Nrf2 (monoclonal, 1:1000, Santa Cruz)

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Indeed, this LDHB-mediated three-step reaction would also explain why NADH production by LDHB-mediated lactate oxidation in the presence of NAD+ decreased in a dose-dependent manner upon addition of CoQ, i.e., the newly formed NADH (step 1) is immediately oxidized to NAD+ (step 2) in the presence of CoQ (Fig